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. 1992 Jan;12(1):42-51.
doi: 10.1016/0888-7543(92)90404-g.

Walking, cloning, and mapping with yeast artificial chromosomes: a contig encompassing D21S13 and D21S16

R Butler  1 , D J Ogilvie, P Elvin, J H Riley, R S Finniear, G Slynn, J E Morten, A F Markham, R Anand
Affiliations

Walking, cloning, and mapping with yeast artificial chromosomes: a contig encompassing D21S13 and D21S16

R Butler et al. Genomics. 1992 Jan.

Abstract

Chromosome 21 has often been used as a model system for the development of genome mapping and cloning strategies in humans. In this report methods for systematic chromosome walking, cloning, and mapping are exemplified in the construction of a 1.5-Mb yeast artificial chromosome (YAC) contig encompassing and extending 400 kb beyond each of the genetic loci D21S13 and D21S16. Isolation of insert-terminal sequences from YACs in this contig provides a set of closely spaced physical markers. These have been used to generate a long-range genomic restriction map.

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