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. 2007 Apr;4(4):315-7.
doi: 10.1038/nmeth1021. Epub 2007 Mar 4.

Single-molecule analysis of DNA-protein complexes using nanopores

Breton Hornblower  1 , Amy Coombs, Richard D Whitaker, Anatoly Kolomeisky, Stephen J Picone, Amit Meller, Mark Akeson
Affiliations

Single-molecule analysis of DNA-protein complexes using nanopores

Breton Hornblower et al. Nat Methods. 2007 Apr.

Abstract

We present a method for rapid measurement of DNA-protein interactions using voltage-driven threading of single DNA molecules through a protein nanopore. Electrical force applied to individual ssDNA-exonuclease I complexes pulls the two molecules apart, while ion current probes the dissociation rate of the complex. Nanopore force spectroscopy (NFS) reveals energy barriers affecting complex dissociation. This method can be applied to other nucleic acid-protein complexes, using protein or solid-state nanopore devices.

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