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. 2007 Apr;4(4):311-3.
doi: 10.1038/nmeth1017. Epub 2007 Mar 4.

High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy

Peter J Verveer  1 , Jim Swoger, Francesco Pampaloni, Klaus Greger, Marco Marcello, Ernst H K Stelzer
Affiliations

High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy

Peter J Verveer et al. Nat Methods. 2007 Apr.

Abstract

We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens.

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  • The rise of the 'projectome'.
    Kasthuri N, Lichtman JW. Kasthuri N, et al. Nat Methods. 2007 Apr;4(4):307-8. doi: 10.1038/nmeth0407-307. Nat Methods. 2007. PMID: 17396125 No abstract available.

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