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Comparative Study
. 2007 May;69(2):371-5.
doi: 10.1016/j.mimet.2007.01.013. Epub 2007 Feb 3.

Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus

Damian C Melles  1 , Willem B van Leeuwen, Susan V Snijders, Deborah Horst-Kreft, Justine K Peeters, Henri A Verbrugh, Alex van Belkum
Affiliations
Comparative Study

Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus

Damian C Melles et al. J Microbiol Methods. 2007 May.

Abstract

We compared multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for typing of Staphylococcus aureus and show that the methods yield similar results, although with differences in resolving power and reproducibility. Epidemiological conditions should determine which is the optimal typing method to be used.

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