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. 1992 Jan;21(1):83-4.
doi: 10.1007/BF00318659.

One-step transformation of yeast in stationary phase

D C Chen  1 , B C Yang, T T Kuo
Affiliations

One-step transformation of yeast in stationary phase

D C Chen et al. Curr Genet. 1992 Jan.

Abstract

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45 degrees C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5 x 10(8) cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 10(4) transformants/microgram plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

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References

    1. J Gen Microbiol. 1986 Nov;132(11):3089-93 - PubMed
    1. Genetics. 1989 May;122(1):19-27 - PubMed
    1. Biotechnology (N Y). 1990 Mar;8(3):223-7 - PubMed
    1. Methods Enzymol. 1990;185:280-97 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 - PubMed

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