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Review
. 2007:421:126-40.
doi: 10.1016/S0076-6879(06)21012-7.

Creating recombination-activated genes and sequence-defined mutant libraries using transposons

Larry Gallagher  1 , Cheri Turner, Elizabeth Ramage, Colin Manoil
Affiliations
Review

Creating recombination-activated genes and sequence-defined mutant libraries using transposons

Larry Gallagher et al. Methods Enzymol. 2007.

Abstract

The properties of a collection of transposon Tn5 derivatives that generate reporter gene fusions and internal protein tags are summarized. Procedures utilizing several of the transposons for generating genes activated by Cre-loxP recombination and for creating large sequence-defined mutant libraries are described in detail.

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