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. 2007 Feb;5(1):75-83.
doi: 10.1089/adt.2006.029.

A high throughput luminescent assay for glycogen synthase kinase-3beta inhibitors

Andrea Baki  1 , Attila Bielik, László Molnár, Györgyi Szendrei, György M Keserü
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A high throughput luminescent assay for glycogen synthase kinase-3beta inhibitors

Andrea Baki et al. Assay Drug Dev Technol. 2007 Feb.

Abstract

A high throughput luminescent assay based on the Kinase-Glo() system (Promega, Madison, WI) has been developed for screening against glycogen synthase kinase-3beta (GSK-3beta). Careful optimization of assay parameters allowed us to develop a robust, reproducible, and sensitive assay. Its usefulness has been demonstrated in a high throughput screening run when screening 55,000 compounds. This campaign yielded five chemical classes of hits, including several highly potent GSK-3beta inhibitors.

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