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. 2007 Mar;15(3):275-9.
doi: 10.1016/j.str.2007.01.016.

Multiprotein expression strategy for structural biology of eukaryotic complexes

Daniel J Fitzgerald  1 , Christiane Schaffitzel, Philipp Berger, Ralf Wellinger, Christoph Bieniossek, Timothy J Richmond, Imre Berger
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Free article

Multiprotein expression strategy for structural biology of eukaryotic complexes

Daniel J Fitzgerald et al. Structure. 2007 Mar.
Free article

Erratum in

  • Structure. 2007 Sep;15(9):1156

Abstract

The concept of the cell as a collection of multisubunit protein machines is emerging as a cornerstone of modern biology, and molecular-level study of these machines in most cases will require recombinant production. Here, we present and validate a strategy to rapidly produce, permutate, and posttranslationally modify large, eukaryotic multiprotein complexes by using DNA recombination in a process that is fully automatable. Parallel production of 12 protein complex variants within a period of weeks resulted in specimens of sufficient quantity and homogeneity for structural biology applications.

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