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. 2007 Mar 28:(12):1218-20.
doi: 10.1039/b618570c. Epub 2007 Jan 26.

Quantitative detection of protein expression in single cells using droplet microfluidics

A Huebner  1 , M Srisa-Art, D Holt, C Abell, F Hollfelder, A J deMello, J B Edel
Affiliations

Quantitative detection of protein expression in single cells using droplet microfluidics

A Huebner et al. Chem Commun (Camb). .

Abstract

We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets; using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.

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