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. 1992 Feb;174(4):1288-92.
doi: 10.1128/jb.174.4.1288-1292.1992.

Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae

C Rensing  1 , U Kües, U Stahl, D H Nies, B Friedrich
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Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae

C Rensing et al. J Bacteriol. 1992 Feb.

Abstract

The gene merA coding for bacterial mercuric ion reductase was cloned under the control of the yeast promoter for alcohol dehydrogenase I in the yeast-Escherichia coli shuttle plasmid pADH040-2 and transformed into Saccharomyces cerevisiae AH22. The resulting transformant harbored stable copies of the merA-containing hybrid plasmid, displayed a fivefold increase in the MIC of mercuric chloride, and synthesized mercuric ion reductase activity.

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