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Comment
. 2007 Mar 6;104(10):3673-4.
doi: 10.1073/pnas.0700276104. Epub 2007 Feb 28.

Meiosis in living color: fluorescence-based tetrad analysis in Arabidopsis

R Scott Hawley  1
Affiliations
Comment

Meiosis in living color: fluorescence-based tetrad analysis in Arabidopsis

R Scott Hawley. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Visualizing meiotic recombination. The segregation of genes encoding different colors of fluorescent proteins enables the detection of recombination events in pollen tetrads produced by Arabidopsis qrt1 mutants. Plants heterozygous for genes encoding red and cyan fluorescent proteins arrayed on the same chromosome in cis (Top Left) shed tetrads with two pollen grains expressing both red and cyan protein (pink after digitally merging images taken using the appropriate filters). Single cross-overs between the markers (Top Right) can be visualized as tetrads with a cyan, a red, a pink, and a nonfluorescent pollen grain (the nonfluorescent grain shows minor background signal). Using three different fluorescent proteins, cyan, yellow, and red, arrayed in cis (Middle Left) allows the detection of double cross-overs (Middle Right), in this case, a four-chromatid double cross-over results in a tetrad with a cyan, a yellow, and cyan (light blue), a red, and a red and yellow (orange) pollen grain. Finally, heterozygous plants with one fluorescent allele and one nonfluorescent mutant allele of yellow fluorescent protein (Bottom Left) can be used to detect gene conversion events (Bottom Right). The tetrads in this figure were digitally positioned for ease of comparison. [I am indebted to Luke Berchowitz and Gregory P. Copenhaver (University of North Carolina, Chapel Hill, NC) for providing this figure.]
See this image and copyright information in PMC

Comment on

References

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