The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae
- PMID: 17361365
- PMCID: PMC6275951
- DOI: 10.2478/s11658-007-0017-y
The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae
Abstract
We compared the oxidation of dihydrorhodamine 123, glutathione contents and activities of superoxide dismutase (SOD) and catalase for three wild-type strains of Saccharomyces cerevisiae grown on media with different carbon sources. The rate of oxidation of dihydrorhodamine 123 was much higher in respiring cells grown on ethanol or glycerol media than in fermenting cells grown on glucose medium. The total SOD activity was highest on glycerol medium and lowest on ethanol medium, while the catalase activity was highest on glycerol medium. The sequence of glutathione content values was: glucose > ethanol > glycerol.
References
-
- Sigler K., Chaloupka J., Brozmanova J., Stadler N., Hofer M. Oxidative stress in microorganisms—I. Microbial vs. higher cells—damage and defenses in relation to cell aging and death. Folia Microbiol. (Praha) 1999;44:587–624. - PubMed
-
- Fiechter A., Gmunder F.K. Metabolic control of glucose degradation in yeast and tumor cells. Adv. Biochem. Eng. Biotechnol. 1989;39:1–28. - PubMed
-
- Shuster J.R. Regulated transcriptional systems for the production of proteins in yeast: regulation by carbon source. Biotechnology. 1989;13:83–108. - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases