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. 1975 Nov;356(11):1671-8.
doi: 10.1515/bchm2.1975.356.2.1671.

Epithelial basement membrane of bovine renal tubuli. Isolation and analysis of the carbohydrate chains

W Ferwerda, W van Dijk

Epithelial basement membrane of bovine renal tubuli. Isolation and analysis of the carbohydrate chains

W Ferwerda et al. Hoppe Seylers Z Physiol Chem. 1975 Nov.

Abstract

The carbohydrate chains present in the tubular basement membrane of bovine kidney were studied. Digestion with collagenase followed with pronase resulted in a complete solubilization of the basement membrane. The different glycopeptides were purified by gel filtration and ion-exchange chromatography. Two kinds of carbohydrate chains could be characterized: oligosaccharides composed of glucosamine, mannose, galactose, fucose and sialic acid, and glucosylgalactose disaccharides. A very small portion of the oligosaccharide chains (ca. 4%) appeared to be free of sialic acid. The bulk of these chains contained sialic acid and fucose, although in small amounts. Only traces of galactosamine were found.

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