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Review
. 2007 May;6(5):755-63.
doi: 10.1128/EC.00008-07. Epub 2007 Mar 16.

Sterol-rich plasma membrane domains in fungi

Francisco J Alvarez  1 , Lois M Douglas, James B Konopka
Affiliations
Review

Sterol-rich plasma membrane domains in fungi

Francisco J Alvarez et al. Eukaryot Cell. 2007 May.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Comparison of filipin and plasma membrane lipid structures. (A) Structure of filipin, a polyene antibiotic that binds sterols. (B) Structure of ergosterol, which contains a four-ring structure common to other sterols, including cholesterol. (C) Sphingolipids are based on a ceramide and contain saturated acyl chains that are variable in length (26 carbon atoms are shown) attached to the sphingosine base. The R group is phosphocholine in the case of sphingomyelin or sugar(s) in the case of the glycosphingolipids. (D) Glycerophospholipids are based on diacylglycerol and typically carry acyl chains of 16 to 18 carbon atoms. One of the acyl chains contains a cis double bond, which prevents close packing with saturated acyl chains present in lipid rafts.
FIG. 2.
FIG. 2.
Examples of filipin staining. (A) S. cerevisiae cells induced with α-factor. (B) S. pombe cells grown to log phase. (C) C. albicans induced to form hyphae with serum. The cells were stained with filipin and then viewed by fluorescence microscopy as previously described (5, 55, 92).
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