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. 2007 Apr;4(4):327-9.
doi: 10.1038/nmeth1020. Epub 2007 Mar 18.

Multiplexed protein detection by proximity ligation for cancer biomarker validation

Simon Fredriksson  1 , William Dixon, Hanlee Ji, Albert C Koong, Michael Mindrinos, Ronald W Davis
Affiliations

Multiplexed protein detection by proximity ligation for cancer biomarker validation

Simon Fredriksson et al. Nat Methods. 2007 Apr.

Abstract

We present a proximity ligation-based multiplexed protein detection procedure in which several selected proteins can be detected via unique nucleic-acid identifiers and subsequently quantified by real-time PCR. The assay requires a 1-microl sample, has low-femtomolar sensitivity as well as five-log linear range and allows for modular multiplexing without cross-reactivity. The procedure can use a single polyclonal antibody batch for each target protein, simplifying affinity-reagent creation for new biomarker candidates.

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