Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Jan;125(1):42-7.
doi: 10.1128/jb.125.1.42-47.1976.

Mutants of Aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase

J R Kinghorn, J A Pateman

Mutants of Aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase

J R Kinghorn et al. J Bacteriol. 1976 Jan.

Abstract

Ten mutants of Aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase (NAD-GDH) have been isolated, and their mutations (gdhB1 through gdhB10) have been shown to lie in the gdhB gene. In addition, a temperature-sensitive gdhB mutant (gdhB11) has been isolated. A revertant (designated R-5) of the mutant gdhB1 bears an additional lesion in the gdhB gene and has altered NAD-GDH activity with altered Km values for ammonia or ammonium ions and for alpha-ketoglutarate. These results suggest that gdhB specifies a structural component for NAD-GDH. The growth characteristics of gdhB mutants indicate the routes by which amino acids are utilized as nitrogen and carbon energy sources. The properties are described of the double mutants bearing the mutations gdhB1 and gdhA1 or tamA119, which have low NADP-GDH activity.

PubMed Disclaimer

References

    1. Adv Genet. 1953;5:141-238 - PubMed
    1. Genet Res. 1965 Nov;6(3):352-9 - PubMed
    1. J Bacteriol. 1973 Jun;114(3):943-50 - PubMed
    1. Mol Gen Genet. 1973 May 9;122(3):261-5 - PubMed
    1. J Gen Microbiol. 1973 Sep;78(1):39-46 - PubMed

LinkOut - more resources