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. 2006 Dec 12;10(B):255-262.

Isolation of genes controlling apoptosis through their effects on cell survival

Gwyn T Williams  1 , Jane P Hughes, Victoria Stoneman, Claire L Anderson, Nicola J McCarthy, Mirna Mourtada-Maarabouni, Mark Pickard, Vanessa L Hedge, Ian Trayner, Farzin Farzaneh
Affiliations

Isolation of genes controlling apoptosis through their effects on cell survival

Gwyn T Williams et al. Gene Ther Mol Biol. .

Abstract

The identification of the most suitable molecular targets for gene and drug therapy is the crucial first step in the development of new disease treatments. The rational identification of such targets depends on a detailed understanding of the pathological changes occuring at the molecular level. We have applied forward genetics approaches to the identification of the critical genes involved in the control of apoptosis in mammalian cells, since defective control of apoptosis underlies many diseases, including cancer and neurodegenerative diseases. We have identified two groups of genes by their effects on cell survival using retroviral cDNA functional expression cloning and retroviral insertional mutagenesis. The identification of these novel genes opens up new areas for apoptosis research and subsequently for the development of new gene and drug therapies.

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Figures

Figure 1
Figure 1
Production of apoptosis-deficient cell clones by retroviral cDNA library functional expression cloning.
Figure 2
Figure 2
Different pathways for production of apoptosis-deficient cell clones by retroviral insertional mutagenesis (RIM).
See this image and copyright information in PMC

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