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. 1992 Feb;11(1):53-9.
doi: 10.1089/hyb.1992.11.53.

Isolation and characterization of a monoclonal anti CK-2 alpha subunit antibody of the IgG1 subclass

I Schmidt-Spaniol  1 , B Boldyreff, O G Issinger
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Isolation and characterization of a monoclonal anti CK-2 alpha subunit antibody of the IgG1 subclass

I Schmidt-Spaniol et al. Hybridoma. 1992 Feb.

Abstract

A monoclonal antibody was produced against the recombinant human alpha subunit of CK-2. The antibody was of the IgG1 subclass and it was isolated from serum-free cell culture media and purified by affinity chromatography on Protein G Sepharose. The antibody can be used to detect specifically the CK-2 alpha subunit in immunoblots from tissue extracts. An ELISA detection test was also established which also allows the identification of the CK-2 alpha subunit.

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