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. 2007 May;45(5):1637-9.
doi: 10.1128/JCM.00382-07. Epub 2007 Mar 21.

Surveillance for reassortant virus by multiplex reverse transcription-PCR specific for eight genomic segments of avian influenza A H5N1 viruses

Prasert Auewarakul  1 , Kantima Sangsiriwut, Kridsda Chaichoune, Arunee Thitithanyanont, Witthawat Wiriyarat, Taweesak Songserm, Rasameepen Ponak-nguen, Jarunee Prasertsopon, Phisanu Pooruk, Pathom Sawanpanyalert, Parntep Ratanakorn, Pilaipan Puthavathana
Affiliations

Surveillance for reassortant virus by multiplex reverse transcription-PCR specific for eight genomic segments of avian influenza A H5N1 viruses

Prasert Auewarakul et al. J Clin Microbiol. 2007 May.

Abstract

Avian influenza H5N1 virus is a global threat. An emergence of a reassortant virus with a pandemic potential is a major concern. Here we describe a multiplex reverse transcription-PCR assay that is specific for the eight genomic segments of the currently circulating H5N1 viruses to facilitate surveillance for a virus resulting from reassortment between human influenza virus and the H5N1 virus.

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Figures

FIG. 1.
FIG. 1.
A representative photograph of multiplex RT-PCR products from an H5N1 virus after electrophoresis in 2% agarose and ethidium bromide staining. Each lane shows products from each tube. The first lane is a 100-bp DNA ladder, and the target gene of each amplification product is labeled on the right.
See this image and copyright information in PMC

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