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Comparative Study
. 2007 May;45(5):1415-9.
doi: 10.1128/JCM.02423-06. Epub 2007 Mar 21.

Comparison of two highly discriminatory molecular fingerprinting assays for analysis of multiple Aspergillus fumigatus isolates from patients with invasive aspergillosis

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Comparative Study

Comparison of two highly discriminatory molecular fingerprinting assays for analysis of multiple Aspergillus fumigatus isolates from patients with invasive aspergillosis

Hanneke A de Valk et al. J Clin Microbiol. 2007 May.

Abstract

Two highly discriminatory fingerprinting assays, short tandem repeat typing and amplified fragment length polymorphism (AFLP), were compared to determine the genetic relatedness between 55 isolates of Aspergillus fumigatus obtained from 15 different patients suffering from proven invasive aspergillosis. Both techniques showed that interpatient isolates belonged to different genotypes and that intrapatient isolates from deep sites were all of the same genotype. By contrast, multiple genotypes were found among isolates originating from respiratory samples. Both techniques have specific advantages and disadvantages. AFLP is more universally applicable, but short tandem repeat analysis offers better discriminatory power and should be the preferred method for standardizing typing of clinical isolates of Aspergillus fumigatus.

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Figures

FIG. 1.
FIG. 1.
Results of both AFLP and STRAf typing on a collection of 55 clinical A. fumigatus isolates. The dendrogram on the left is based on the AFLP fingerprints. The indicator lines above the traces represent invariable bands, whereas the indicator lines below the traces represent variable bands in the AFLP fingerprints. After each fingerprint are the typing results for the nine markers of the STRAf panel. The dendrogram on the right is based on the STRAf results. Types were assigned arbitrarily, starting with the oldest isolate. The scale bars indicate the percentages of similarity. Sample numbers correspond to those in Table 1.

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