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. 1992 Mar;66(3):1432-41.
doi: 10.1128/JVI.66.3.1432-1441.1992.

Functional characterization of a U5 ribozyme: intracellular suppression of human immunodeficiency virus type 1 expression

B Dropulić  1 , N H Lin, M A Martin, K T Jeang
Affiliations

Functional characterization of a U5 ribozyme: intracellular suppression of human immunodeficiency virus type 1 expression

B Dropulić et al. J Virol. 1992 Mar.

Abstract

We have designed a ribozyme that cleaves human immunodeficiency virus type 1 (HIV-1) RNA in U5 (at nucleotide +115). This ribozyme was tested in vitro and was found to give efficient and specific digestion of RNA containing the HIV-1 U5 sequence. When the U5 ribozyme was placed into the HIV-1 genome, virus replication was suppressed in tissue culture. Introduction of this ribozyme into cells by using an amphotropic retrovirus vector significantly reduced expression of U5-containing RNA in cells chronically infected with HIV-1. Naive T cells were cocultivated with packaging cells that produce defective amphotropic retroviruses containing the U5 ribozyme. These lymphocytes were found to be partially protected from HIV-1 infection.

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