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. 2007 Jun;45(6):2061-3.
doi: 10.1128/JCM.00136-07. Epub 2007 Mar 28.

Rates of and reasons for failure of commercial human immunodeficiency virus type 1 viral load assays in Brazil

Jan Felix Drexler  1 , Luciano Kleber de Souza Luna, Celia Pedroso, Diana Brasil Pedral-Sampaio, Artur T L Queiroz, Carlos Brites, Eduardo M Netto, Christian Drosten
Affiliations

Rates of and reasons for failure of commercial human immunodeficiency virus type 1 viral load assays in Brazil

Jan Felix Drexler et al. J Clin Microbiol. 2007 Jun.

Abstract

We examined failures of commercial human immunodeficiency virus type 1 (HIV-1) viral load assays of 1,195 plasma samples from Brazilian patients. Assay failure was assumed for samples in which the virus was undetectable by commercial assay but which tested positive by real-time reverse transcription-PCR of the HIV-1 long terminal repeat (LTR) region or if the viral load differed by >2 log10 from that determined by LTR assay. Failure rates for Bayer Versant bDNA 3.0, Roche Amplicor Monitor v1.5, and bioMerieux NucliSens QT were 0.68, 0.47, and 4.33%, respectively. NucliSens may be inadequate for use in Brazil.

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Figures

FIG. 1.
FIG. 1.
Nucleotide mismatches of Amplicor Monitor v1.5 (A) and NucliSens HIV-1 QT (B) assays at oligonucleotide binding sites in the gag p24 gene. The viral loads in samples HU-EFS and HI-LRM were underquantified by Monitor v1.5. The viral loads in samples 13996 to 14423 were underquantified by NucliSens. Samples RS310 to RS481 were underquantified by bDNA v3.0. Primer and probe sequences were obtained from N. L. Michael et al. (9) and C. Christopherson et al. (5) for Monitor v1.5 and B. van Gemen et al. (16) and I. von Truchsess et al. (18) for NucliSens, respectively.
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