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. 1992 Feb;65(2):234-8.
doi: 10.1038/bjc.1992.47.

Molecular and functional characterisation of a fusion protein suited for tumour specific prodrug activation

K Bosslet  1 , J Czech, P Lorenz, H H Sedlacek, M Schuermann, G Seemann
Affiliations
Free PMC article

Molecular and functional characterisation of a fusion protein suited for tumour specific prodrug activation

K Bosslet et al. Br J Cancer. 1992 Feb.
Free PMC article

Abstract

A fusion protein consisting of the humanised Fab fragment of the anti CEA MAb BW 431 and the human beta-glucuronidase was expressed in BHK cells. Functional testing revealed that the specificity and avidity of the humanised V region was similar to the original murine MAb BW 431. Furthermore, the enzymatic activity, pH sensitivity and stability of the human beta-glucuronidase in the fusion protein was comparable to the activity of recombinant human beta-glucuronidase. Using anti-idiotype affinity chromatography, two molecules of a molecular weight of 125 kDa or 250 kDa could be visualized under nonreducing conditions in SDS-PAGE. Reducing conditions revealed a 25 kDa light and 100 kDa heavy chain. Due to its suitable biological characteristics this fusion protein might be an appropriate molecule allowing a site specific antibody directed enzyme prodrug therapy (ADEPT) in vivo.

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References

    1. Br J Cancer. 1990 May;61(5):659-62 - PubMed
    1. Nature. 1986 May 29-Jun 4;321(6069):522-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4045-9 - PubMed
    1. Biochemistry. 1978 Feb 7;17(3):385-91 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed

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