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. 1977 May 1;163(2):247-51.
doi: 10.1042/bj1630247.

The interaction of N-acetylhexosaminidase with insolubilized concanavalin A

M G Brattain, P M Kimball, T G Pretlow 2nd, M E Marks

The interaction of N-acetylhexosaminidase with insolubilized concanavalin A

M G Brattain et al. Biochem J. .

Abstract

The specific interaction between human N-acetylhexosaminidase and concanavalin A was evaluated with respect to temperature, time, pH and concentration of specific ligand in incubation mixtures containing the enzyme and insolubilized lectin. Elution of the enzyme from insolubilized concanavalin A is dependent on both temperature and concentration of alpha-methyl mannoside. Conditions for a high yield of the enzyme from chromatography on insolubilized concanavalin A are described.

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