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. 2007 Apr 25;129(16):4874-5.
doi: 10.1021/ja070200b. Epub 2007 Mar 31.

Lithographic patterning of photoreactive cell-adhesive proteins

Isaac S Carrico  1 , Stacey A Maskarinec, Sarah C Heilshorn, Marissa L Mock, Julie C Liu, Paul J Nowatzki, Christian Franck, Guruswami Ravichandran, David A Tirrell
Affiliations

Lithographic patterning of photoreactive cell-adhesive proteins

Isaac S Carrico et al. J Am Chem Soc. .
No abstract available

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Figures

Figure 1
Figure 1
Characteristics of aECM-N3 proteins. (A) Primary sequences of aECM-N3 variants. (B) FTIR demonstrates loss of the characteristic azide asymmetric stretch as a function of irradiation time of CS5-N3 films. (C) Peak area vs. irradiation time yields a first order decay with t1/2=34 sec. (D) Uniaxial tensile testing of irradiated mold-cast films.
Figure 2
Figure 2
Confocal microscopy of Rat-1 fibroblasts attached to photopatterned RGD-N3. Immunostaining with anti-T7 (blue) demonstrates colocalization of aECM-N3 protein and cells (stained with rhodamine phalloidin (yellow)). Scale bars represent 100 μm.
Figure 3
Figure 3
Rat-1 fibroblast cell spread areas on (A) fibronectin, (B) BSA, (C) RGD-N3, and (D) RDG-N3. RGD-N3 supports sequence-specific cell spreading.
See this image and copyright information in PMC

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