doi: 10.1016/j.febslet.2007.03.040.
Epub 2007 Mar 28.
Sequence effects of single base loops in intramolecular quadruplex DNA
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- PMID: 17399710
- DOI: 10.1016/j.febslet.2007.03.040
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Sequence effects of single base loops in intramolecular quadruplex DNA
FEBS Lett.
.
Free article
Abstract
We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1',2'-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2 degrees C). Quadruplexes containing loops with single A residues were less stable by 8 degrees C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability.
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