Influence of disk preparation on detection of metallo-beta-lactamase-producing isolates by the combined disk assay
- PMID: 17409202
- PMCID: PMC1933093
- DOI: 10.1128/JCM.02467-06
Influence of disk preparation on detection of metallo-beta-lactamase-producing isolates by the combined disk assay
Abstract
The combined disk assay has been used for detection of metallo-beta-lactamase-producing isolates. We have observed that the size of inhibition zones produced by many beta-lactam/metallo-beta-lactamase inhibitor (IMBL) combinations may differ depending on the way that the combined disks were prepared. Among the 10 beta-lactam/IMBL combinations tested, only the imipenem/EDTA combination produced similar results.
References
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- Castanheira, M., R. C. Picao, R. E. Mendes, A. C. Pignatari, H. S. Sader, and A. C. Gales. 2006. Discrepancy in the metallo-beta-lactamase phenothypic tests results associated with diversity in the promoter region of class 1 integrons. Abstr. 16th Eur. Cong. Clin. Microbiol. Infect. Dis., European Society of Microbiology and Infectious Diseases, Nice, France.
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