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. 2007 Jun;45(6):2058-60.
doi: 10.1128/JCM.02467-06. Epub 2007 Apr 4.

Influence of disk preparation on detection of metallo-beta-lactamase-producing isolates by the combined disk assay

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Influence of disk preparation on detection of metallo-beta-lactamase-producing isolates by the combined disk assay

Soraya S Andrade et al. J Clin Microbiol. 2007 Jun.

Abstract

The combined disk assay has been used for detection of metallo-beta-lactamase-producing isolates. We have observed that the size of inhibition zones produced by many beta-lactam/metallo-beta-lactamase inhibitor (IMBL) combinations may differ depending on the way that the combined disks were prepared. Among the 10 beta-lactam/IMBL combinations tested, only the imipenem/EDTA combination produced similar results.

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References

    1. Arakawa, Y., N. Shibata, K. Shibayama, H. Kurokawa, T. Yagi, H. Fujiwara, and M. Goto. 2000. Convenient test for screening metallo-β-lactamase-producing gram-negative bacteria by using thiol compounds. J. Clin. Microbiol. 38:40-43. - PMC - PubMed
    1. Castanheira, M., R. E. Mendes, R. C. Picao, F. P. Pinto, A. M. O. Machado, T. R. Walsh, and A. C. Gales. 2006. Genetic analysis of a multidrug-resistant (MDR) Enterobacter cloacae producing IMP-1 metallo-β-lactamase (MBL). Abstr. 46th Intersci. Conf. Antimicrob. Agents Chemother., American Society for Microbiology, Washington, DC.
    1. Castanheira, M., R. C. Picao, R. E. Mendes, A. C. Pignatari, H. S. Sader, and A. C. Gales. 2006. Discrepancy in the metallo-beta-lactamase phenothypic tests results associated with diversity in the promoter region of class 1 integrons. Abstr. 16th Eur. Cong. Clin. Microbiol. Infect. Dis., European Society of Microbiology and Infectious Diseases, Nice, France.
    1. Castanheira, M., M. A. Toleman, R. N. Jones, F. J. Schmidt, and T. R. Walsh. 2004. Molecular characterization of a β-lactamase gene, blaGIM-1, encoding a new subclass of metallo-β-lactamase. Antimicrob. Agents Chemother. 48:4654-4661. - PMC - PubMed
    1. Franklin, C., L. Liolios, and A. Y. Peleg. 2006. Phenotypic detection of carbapenem-susceptible metallo-β-lactamase-producing gram-negative bacilli in the clinical laboratory. J. Clin. Microbiol. 44:3139-3144. - PMC - PubMed

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