Effect of Xenopus laevis oocyte extract on supercoiled simian virus 40 DNA: formation of complex DNA
- PMID: 174111
- PMCID: PMC335948
- DOI: 10.1073/pnas.73.2.554
Effect of Xenopus laevis oocyte extract on supercoiled simian virus 40 DNA: formation of complex DNA
Abstract
A soluble cell-free extract derived from stage 6 Xenopus laevis oocytes is described. From supercoiled simian virus 40 DNA the extract produces nicked circles (having a single-strand scission), linear molecules of full unit size, shorter length fragments, and various forms of complex DNA.
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