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. 1992 Feb 11;20(3):433-8.
doi: 10.1093/nar/20.3.433.

Purification and properties of the MboII, a class-IIS restriction endonuclease

M Sektas  1 , T Kaczorowski, A J Podhajska
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Free PMC article

Purification and properties of the MboII, a class-IIS restriction endonuclease

M Sektas et al. Nucleic Acids Res. .
Free PMC article

Abstract

After five purification steps a homogeneous preparation of endonuclease MboII was obtained, and several properties of the enzyme were determined. MboII is a monomer, with Mr under native and denaturing conditions being 47-49 x 10(3) Da. Endonuclease MboII is a basic protein (pI 8.3) which remains active when Mg2+ is replaced by Mn2+, Co2+, Ca2+, or Fe2+. MboII exhibits a star activity in the presence of some of the following reagents or ions: DMSO, glycerol, ethanol (and Co2+ or Mn2+ at pH 6). MboII does not bend DNA and is heat sensitive, losing activity after 15 min at 50 degrees C.

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References

    1. J Biol Chem. 1961 May;236:1372-9 - PubMed
    1. Gene Amplif Anal. 1987;5:185-207 - PubMed
    1. Gene. 1991 Apr;100:13-26 - PubMed
    1. Nucleic Acids Res. 1989 Aug 25;17(16):6515-22 - PubMed
    1. Mol Gen Genet. 1987 Oct;209(3):570-4 - PubMed

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