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. 1992 Feb 11;20(3):445-7.
doi: 10.1093/nar/20.3.445.

Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy

E Henderson  1
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Free PMC article

Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy

E Henderson. Nucleic Acids Res. .
Free PMC article

Erratum in

  • Nucleic Acids Res 1992 Apr 11;20(7):1841

Abstract

The atomic force microscope (AFM) was used to image supercoiled plasmid DNA deposited on a mica surface in either a hydrated or desiccated state. Hydrated plasmid was precisely cut by the scanning tip at a location determined by the instrument operator. Small pieces of DNA (100-150 nm in length) were excised and deposited adjacent to the dissected plasmid, demonstrating that it is possible to remove and manipulate genomic DNA fragments, unresolvable by light microscopy, from defined chromosomal locations by AFM.

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