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. 2007 May;4(5):409-12.
doi: 10.1038/nmeth1040. Epub 2007 Apr 8.

A genomic integration method to visualize localization of endogenous mRNAs in living yeast

Liora Haim  1 , Gadi Zipor, Stella Aronov, Jeffrey E Gerst
Affiliations

A genomic integration method to visualize localization of endogenous mRNAs in living yeast

Liora Haim et al. Nat Methods. 2007 May.

Abstract

mRNA localization may be an important determinant for protein localization. We describe a simple PCR-based genomic-tagging strategy (m-TAG) that uses homologous recombination to insert binding sites for the RNA-binding MS2 coat protein (MS2-CP) between the coding region and 3' untranslated region (UTR) of any yeast gene. Upon coexpression of MS2-CP fused with GFP, we demonstrate the localization of endogenous mRNAs (ASH1, SRO7, PEX3 and OXA1) in living yeast (Saccharomyces cerevisiae).

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