A rRNA-mRNA base pairing model for UGA-dependent termination
- PMID: 1742356
- DOI: 10.1016/0300-9084(91)90155-t
A rRNA-mRNA base pairing model for UGA-dependent termination
Abstract
A series of site-directed mutations has been constructed in E coli 16S rRNA and shown to suppress UGA-dependent translational termination. With the exception of the C726 to G base change, all were constructed in helix 34. Characterization of these mutations is reviewed here and from these data and mRNA-rRNA base pairing model for the termination event is presented. The interaction functions via antiparallel base pairing between either 1 of the 2 UCA motifs in helix 34 and the complementary UGA stop codon on the message, thus forming a quasicontinuous A-type helical structure that is further stabilized by stacking enthalpy. Finally, rRNA motifs potentially required for UAA and UAG-dependent translational termination are discussed.
Similar articles
-
Mutations in 16S rRNA that affect UGA (stop codon)-directed translation termination.Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6603-7. doi: 10.1073/pnas.88.15.6603. Proc Natl Acad Sci U S A. 1991. PMID: 1907372 Free PMC article.
-
The involvement of base 1054 in 16S rRNA for UGA stop codon dependent translational termination.Nucleic Acids Res. 1990 Oct 11;18(19):5625-32. doi: 10.1093/nar/18.19.5625. Nucleic Acids Res. 1990. PMID: 2216755 Free PMC article.
-
UGA suppression by a mutant RNA of the large ribosomal subunit.Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12309-13. doi: 10.1073/pnas.92.26.12309. Proc Natl Acad Sci U S A. 1995. PMID: 8618891 Free PMC article.
-
Termination of protein synthesis.Mol Biol Rep. 1994 May;19(3):171-81. doi: 10.1007/BF00986959. Mol Biol Rep. 1994. PMID: 7969105 Review.
-
Ribosomal RNAs in translation termination: facts and hypotheses.Biochemistry (Mosc). 1999 Dec;64(12):1354-9. Biochemistry (Mosc). 1999. PMID: 10648958 Review.
Cited by
-
rRNA-like sequences occur in diverse primary transcripts: implications for the control of gene expression.Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):422-7. doi: 10.1073/pnas.94.2.422. Proc Natl Acad Sci U S A. 1997. PMID: 9012798 Free PMC article.
-
The identity of the base following the stop codon determines the efficiency of in vivo translational termination in Escherichia coli.EMBO J. 1995 Jan 3;14(1):151-8. doi: 10.1002/j.1460-2075.1995.tb06985.x. EMBO J. 1995. PMID: 7828587 Free PMC article.
-
Two regions of the Escherichia coli 16S ribosomal RNA are important for decoding stop signals in polypeptide chain termination.Nucleic Acids Res. 1993 May 11;21(9):2109-15. doi: 10.1093/nar/21.9.2109. Nucleic Acids Res. 1993. PMID: 8502551 Free PMC article.
-
The translational function of nucleotide C1054 in the small subunit rRNA is conserved throughout evolution: genetic evidence in yeast.Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2517-22. doi: 10.1073/pnas.93.6.2517. Proc Natl Acad Sci U S A. 1996. PMID: 8637906 Free PMC article.
-
Mutations in E.coli 16s rRNA that enhance and decrease the activity of a suppressor tRNA.Nucleic Acids Res. 1992 Apr 11;20(7):1567-71. doi: 10.1093/nar/20.7.1567. Nucleic Acids Res. 1992. PMID: 1374555 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
