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Comparative Study
. 2007 May 2;129(17):5344-5.
doi: 10.1021/ja070567g. Epub 2007 Apr 11.

Biophysical characterization of a beta-peptide bundle: comparison to natural proteins

E James Petersson  1 , Cody J Craig, Douglas S Daniels, Jade X Qiu, Alanna Schepartz
Affiliations
Comparative Study

Biophysical characterization of a beta-peptide bundle: comparison to natural proteins

E James Petersson et al. J Am Chem Soc. .
No abstract available

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Figures

Figure 1
Figure 1
(A) Helical net representation of the Zwit-1F monomer. β3-Amino acids are designated by the single letter corresponding to the equivalent α-amino acid. O signifies ornithine. (B) Zwit-1F octamer structure determined by X-ray crystallography. (C) Plot of MRE205 as a function of [Zwit-1F] fit to a monomer–octamer equilibrium. Inset: CD spectra (MRE in units of 103 deg·cm2·dmol−1) at the indicated [Zwit-1F] (μM).
Figure 2
Figure 2
(A) Temperature-dependent CD analysis of Zwit-1F. Plot of MRE205 as a function of temperature at the indicated Zwit-1F concentration (μM). (B) DSC analysis of Zwit-1F unfolding fit to a subunit dissociation model. Raw data are shown as black circles.
Figure 3
Figure 3
(A) 500 MHz 1H NMR spectra of 1.5 mM Zwit-1F, acquired in phosphate-buffered “H2O” (9:1 H2O/D2O) or at the indicated times after reconstitution of a lyophilized Zwit-1F sample in phosphate-buffered D2O. (B) Peak heights of the indicated resonances (normalized to the peak at 6.70 ppm) fit to exponential decays. Bars indicate standard error.
See this image and copyright information in PMC

References

    1. Daniels DS, Petersson EJ, Qiu XJ, Schepartz A. J Am Chem Soc. 2007;129:1532–1533. - PMC - PubMed

    1. CD extrema between 205 and 215 nm have been attributed previously to the 314-helix. For details, see: Seebach D, Beck AK, Bierbaum DJ. Chem Biodiversity. 2004;1:1111–1239.

    1. See Supporting Information.

    1. The MRE205 concentration dependence of Zwit-1F also fits a monomer–hexamer equilibrium, as did previous AU data. Although the Zwit-1F X-ray structure shows an octamer, we cannot currently exclude the presence of hexameric forms in solution.

    1. Baxevanis AD, Godfrey JE, Moudrianakis EN. Biochemistry. 1991;30:8817–8823. - PubMed

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