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Review
. 2007 Apr;20(2):280-322.
doi: 10.1128/CMR.00033-06.

Helicobacter pylori detection and antimicrobial susceptibility testing

Francis Mégraud  1 , Philippe Lehours
Affiliations
Review

Helicobacter pylori detection and antimicrobial susceptibility testing

Francis Mégraud et al. Clin Microbiol Rev. 2007 Apr.

Abstract

The discovery of Helicobacter pylori in 1982 was the starting point of a revolution concerning the concepts and management of gastroduodenal diseases. It is now well accepted that the most common stomach disease, peptic ulcer disease, is an infectious disease, and all consensus conferences agree that the causative agent, H. pylori, must be treated with antibiotics. Furthermore, the concept emerged that this bacterium could be the trigger of various malignant diseases of the stomach, and it is now a model for chronic bacterial infections causing cancer. Most of the many different techniques involved in diagnosis of H. pylori infection are performed in clinical microbiology laboratories. The aim of this article is to review the current status of these methods and their application, highlighting the important progress which has been made in the past decade. Both invasive and noninvasive techniques will be reviewed.

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Figures

FIG. 1.
FIG. 1.
Helicobacter pylori observed on a gastric biopsy smear after acridine orange staining. Magnification, ×1,000.
FIG. 2.
FIG. 2.
Helicobacter heilmannii observed on a gastric biopsy histological preparation after hematoxylin-eosin staining. Magnification, ×1,000.
FIG. 3.
FIG. 3.
Mutations arising in domain V of Helicobacter pylori 23S rRNA and conferring resistance to clarithromycin.
See this image and copyright information in PMC

References

    1. Abdalla, A. M., E. M. Sordillo, Z. Hanzely, G. I. Perez Perez, M. J. Blaser, P. R. Holt, and S. F. Moss. 1998. Insensitivity of the CLOtest for H. pylori, especially in the elderly. Gastroenterology 115:243-244. - PubMed
    1. Adachi, K., A. Kawamura, M. Ono, K. Masuzaki, T. Takashima, M. Yuki, H. Fujishiro, S. Ishihara, and Y. Kinoshita. 2002. Comparative evaluation of urine-based and other minimally invasive methods for the diagnosis of Helicobacter pylori infection. J. Gastroenterol. 37:703-708. - PubMed
    1. Agha, A., A. R. Opekun, S. Abudayyeh, and D. Y. Graham. 2005. Effect of different organic acids (citric, malic and ascorbic) on intragastric urease activity. Aliment. Pharmacol. Ther. 21:1145-1148. - PubMed
    1. Agha-Amiri, K., U. Peitz, D. Mainz, S. Kahl, A. Leodolter, and P. Malfertheiner. 2001. A novel immunoassay based on monoclonal antibodies for the detection of Helicobacter pylori antigens in human stool. Z. Gastroenterol. 39:555-560. - PubMed
    1. Alarcon, T., D. Domingo, N. Prieto, and M. Lopez Brea. 2000. PCR using 3′-mismatched primers to detect A2142C mutation in 23S rRNA conferring resistance to clarithromycin in Helicobacter pylori clinical isolates. J. Clin. Microbiol. 38:923-925. - PMC - PubMed

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