[Conditions for splitting protodioscine--the main glycoside from Tribulus terrestris L. by the enzymatic preparation from Aspergillus niger BKMt-33]

Abstract

The conditions for splitting protodioscine--the main steroid saponine isolated from Tribulus terrestris L. by the enzymic preparation of Aspergillus niger str. BKMt-33 were investigated. The optimal conditions were found to be as follows: pH 4-5, temperature 30-37 degrees (the substrate concentration--5 mg%, concentration of the enzymic preparation--1%). Under these conditions the enzymolysis continued 24 hours. Mg+2 and K+ ions accelerated the reaction twice. As a result of the enzymic hydrolysis dioscine and trilline were obtained. This indicates beta-glucosidase and alpha-rhamnosidase activities of the enzymic complex isolated from Aspergillus niger str. BKMt-33.