[Study of organ cultures of human embryonic intestines infected with the human cytomegalovirus and the Japanese encephalitis virus]

Abstract

Reproduction of human cytomegalovirus (CMV) and Japanese encephalitis virus (JE) was studied in organ cultures of human embryo intestine under conditions of mono- and combined infection. The titer of JE virus in monoinfection reached its maximum level (10(7) TCD50/ml) whereas the titer of CMV did not exceed 10(2) TCD50/ml. In combined infection the titer of CMV increased to 10(5.5) TCD50/ml and the titer of JE virus remained at the same level (10(7) TCD50/ml). The results obtained in light and scanning electron microscopy studies indicate that in monoinfection CMV causes considerably earlier and more profound degenerative changes in mesanchymal elements of embryonal intestinal tissues than does JE virus. At the same time the combined infection of organ cultures with these viruses is not cytocidal.