[Purification and characterization of extracellular halophilic protease from haloarchaea Natrinema sp. R6-5]
[Article in
Chinese]
Affiliations
- PMID: 17436645
Item in Clipboard
[Purification and characterization of extracellular halophilic protease from haloarchaea Natrinema sp. R6-5]
[Article in
Chinese]
Wei Sheng Wu Xue Bao.
2007 Feb.
Abstract
A halophilic extracellular protease from a halophilic archaea Natrinema sp. R6-5 was purified to SDS-PAGE homogeneity using bacitracin-Sepharose 4B chromatography. A molecular mass of the purified protease subunit was 62KD determined by SDS-PAGE. The protease activity was inhibited by phenylmethylsulfonyl fluoride (PMSF), suggesting that the protease belong to serine protease. The protease exhibited optimum NaCl concentration is 3 mol/L. At the 3 mol/L NaCl concentration, the optimum temperature and the optimum pH were 45 degrees C and 8.0. The protease could keep high activity and stability in high salt environment and had potential application value.
Similar articles
-
Optimization of culture conditions for the production of haloalkaliphilic thermostable protease from an extremely halophilic archaeon Halogeometricum sp. TSS101.Lett Appl Microbiol. 2006 Oct;43(4):385-91. doi: 10.1111/j.1472-765X.2006.01980.x. Lett Appl Microbiol. 2006. PMID: 16965368
-
Purification and characterization of a protease produced by an aerobic haloalkaliphilic species belonging to the Salinivibrio genus.Res Microbiol. 2005 May;156(4):478-84. doi: 10.1016/j.resmic.2004.12.004. Epub 2005 Feb 19. Res Microbiol. 2005. PMID: 15862445
-
Partial purification and characterization of extracellular protease from a halophilic and thermotolerant strain Streptomyces pseudogrisiolus NRC-15.Indian J Biochem Biophys. 2013 Aug;50(4):305-11. Indian J Biochem Biophys. 2013. PMID: 24772950
-
Operative utility of salt-stable proteases of halophilic and halotolerant bacteria in the biotechnology sector.Int J Biol Macromol. 2018 Oct 1;117:493-522. doi: 10.1016/j.ijbiomac.2018.05.217. Epub 2018 May 30. Int J Biol Macromol. 2018. PMID: 29857102 Review.
-
Recent advances in the production, properties and applications of haloextremozymes protease and lipase from haloarchaea.World J Microbiol Biotechnol. 2023 Sep 27;39(11):322. doi: 10.1007/s11274-023-03779-x. World J Microbiol Biotechnol. 2023. PMID: 37755613 Review.