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. 1991;117(6):561-7.
doi: 10.1007/BF01613289.

Killing of Burkitt-lymphoma-derived Daudi cells by ultraviolet-inactivated vaccinia virus

L Grunwald-Beard  1 , H Gamliel, G Parag, S Vedantham, Z Zakay-Rones
Affiliations

Killing of Burkitt-lymphoma-derived Daudi cells by ultraviolet-inactivated vaccinia virus

L Grunwald-Beard et al. J Cancer Res Clin Oncol. 1991.

Abstract

Interaction of active and UV-inactivated vaccinia virus at high multiplicity caused cytological changes and inhibition in cellular protein and DNA synthesis, thus arresting the multiplication of Burkitt-lymphoma-derived Daudi cells and eventually killing the cells. Adsorption to the cells but the lack of penetration was evident by immunofluorescence, electron microscopy and [3H]thymidine-labeled virus incorporation. Viral DNA synthesis or virus replication was not demonstrated. Thus, it appears that the massive adsorption of viral particles, active or UV-inactivated, or possibly a "toxic" component that resides in the virion, damages the plasma membrane and may be responsible for killing the cells by a mechanism of lysis from without.

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