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. 2007 Jun;45(6):1874-83.
doi: 10.1128/JCM.00223-07. Epub 2007 Apr 18.

Identification of genotypically diverse Cryptococcus neoformans and Cryptococcus gattii isolates by Luminex xMAP technology

M Bovers  1 , M R Diaz, F Hagen, L Spanjaard, B Duim, C E Visser, H L Hoogveld, J Scharringa, I M Hoepelman, J W Fell, T Boekhout
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Identification of genotypically diverse Cryptococcus neoformans and Cryptococcus gattii isolates by Luminex xMAP technology

M Bovers et al. J Clin Microbiol. 2007 Jun.

Abstract

A Luminex suspension array, which had been developed for identification of Cryptococcus neoformans and Cryptococcus gattii isolates, was tested by genotyping a set of 58 mostly clinical isolates. All genotypes of C. neoformans and C. gattii were included. In addition, cerebrospinal fluid (CSF) obtained from patients with cryptococcal meningitis was used to investigate the feasibility of the technique for identification of the infecting strain. The suspension array correctly identified haploid isolates in all cases. Furthermore, hybrid isolates possessing two alleles of the Luminex probe region could be identified as hybrids. In CSF specimens, the genotype of the cryptococcal strains responsible for infection could be identified after optimization of the PCR conditions. However, further optimization of the DNA extraction protocol is needed to enhance the usability of the method in clinical practice.

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Figures

FIG. 1.
FIG. 1.
Results obtained with the Luminex suspension array for all six haploid groups within C. neoformans and C. gattii. Examples of the results obtained with the AFLP1, -2, -4, -5, -6, and -7 genotypic groups are depicted in panels a, b, c, d, e, and f, respectively.
FIG. 2.
FIG. 2.
Results obtained with the Luminex suspension array for all of the serotype AD (AFLP3) hybrids of C. neoformans. Panel a shows five serotype AD hybrids that were identified by three probes, and panel b shows two serotype AD hybrids that were identified by two probes.
FIG. 3.
FIG. 3.
Results obtained with the Luminex suspension array for the serotype BD (AFLP8) hybrid isolates.
FIG. 4.
FIG. 4.
Luminex suspension array results for the amplicons obtained from CSF specimens.
See this image and copyright information in PMC

References

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