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. 2007 Apr;8(4):221-7.
doi: 10.1631/jzus.2007.B0221.

Proteomic analysis of the serum in patients with idiopathic pulmonary arterial hypertension

Min Yu  1 , Xing-xiang Wang, Fu-rong Zhang, Yun-peng Shang, Yu-xi Du, Hong-juan Chen, Jun-zhu Chen
Affiliations

Proteomic analysis of the serum in patients with idiopathic pulmonary arterial hypertension

Min Yu et al. J Zhejiang Univ Sci B. 2007 Apr.

Abstract

Idiopathic pulmonary arterial hypertension (IPAH) is a rare disease of unknown etiology. The exact pathogenesis of pulmonary arterial hypertension is still not well known. In the past decades, many protein molecules have been found to be involved in the development of IPAH. With proteomic techniques, profiling of human plasma proteome becomes more feasible in searching for disease-related markers. In present study, we showed the protein expression profiles of the serum of IPAH and healthy controls after depleting a few high-abundant proteins in serum. Thirteen spots had changed significantly in IPAH compared with healthy controls and were identified by LC-MS/MS. Alpha-1-antitrypsin and vitronectin were down-regulated in IPAH and may be valuable candidates for further explorations of their roles in the development of IPAH.

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Figures

Fig. 1
Fig. 1
Representative 2-DE maps of serum proteins. Proteins were separated by 2-DE and visualized by silver staining. Two hundred micrograms of protein was loaded on 24 cm, non-linear IPG strips of pH 3~10 for first dimension electrophoresis, followed by vertical 12.5% SDS-PAGE for second dimension electrophoresis. (a) Healthy controls; (b) IPAH patients
Fig. 1
Fig. 1
Representative 2-DE maps of serum proteins. Proteins were separated by 2-DE and visualized by silver staining. Two hundred micrograms of protein was loaded on 24 cm, non-linear IPG strips of pH 3~10 for first dimension electrophoresis, followed by vertical 12.5% SDS-PAGE for second dimension electrophoresis. (a) Healthy controls; (b) IPAH patients
Fig. 2
Fig. 2
Representative protein spot identified with LC-MS/MS. (a) Base peak chromatogram of spot 1; (b) Fragmentation spectrum of the ion with m/z 918.74 from a tryptic peptide of spot 1. The identified sequence is VFSNGADLSGVTEEAPLK interpreted as y series and b series ions. The spot was identified as alpha-1-antitrypsin
Fig. 2
Fig. 2
Representative protein spot identified with LC-MS/MS. (a) Base peak chromatogram of spot 1; (b) Fragmentation spectrum of the ion with m/z 918.74 from a tryptic peptide of spot 1. The identified sequence is VFSNGADLSGVTEEAPLK interpreted as y series and b series ions. The spot was identified as alpha-1-antitrypsin
See this image and copyright information in PMC

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