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Comment
. 2007 May;15(5):846-8.
doi: 10.1038/sj.mt.6300166.

Programming the next generation of dendritic cells

Richard G Carroll  1 , Carl H June
Affiliations
Comment

Programming the next generation of dendritic cells

Richard G Carroll et al. Mol Ther. 2007 May.
No abstract available

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Figures

Figure 1
Figure 1. Possible applications of programmed DCs
A bone marrow sample obtained from a normal donor or a tumor-bearing patient is placed in culture, and DC precursors are transduced with a cocktail of lentiviral vectors to express cytokines GM-CSF and IL-4 as well as tumor antigen(s) (TAA). (a) In the approach used by Koya et al., a clinical application would be to use the transduced cell population for prophylactic or therapeutic tumor vaccine by injection of engineered DCs by various routes. (b) An ex vivo application would be to use the engineered DCs to prime tumor-reactive T cells for later adoptive transfer therapy. In this scenario it is likely that maturation and activation (e.g., CD40L stimulation) of the DCs would enhance the efficiency. (c) A humanized mouse approach could be used to generate populations of tumor-reactive T cells as a source of tumor-specific T cells or, conceivably, to generate T cells for therapeutic use by engrafting mice with CD34 cells or cord blood T cells, and using the engineered DCs to induce the outgrowth of large numbers of tumor-reactive human T cells. TCRs, T-cell receptors. (d) Direct administration of lentiviral vectors may result in in situ programming of DC precursor cells in vivo. Ag, antigen.
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References

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