Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007;2(4):792-6.
doi: 10.1038/nprot.2007.105.

A method for genetic modification of human embryonic stem cells using electroporation

Magdaline Costa  1 , Mirella Dottori, Koula Sourris, Pegah Jamshidi, Tanya Hatzistavrou, Richard Davis, Lisa Azzola, Steven Jackson, Sue Mei Lim, Martin Pera, Andrew G Elefanty, Edouard G Stanley
Affiliations

A method for genetic modification of human embryonic stem cells using electroporation

Magdaline Costa et al. Nat Protoc. 2007.

Abstract

The ability to genetically modify human embryonic stem cells (HESCs) will be critical for their widespread use as a tool for understanding fundamental aspects of human biology and pathology and for their development as a platform for pharmaceutical discovery. Here, we describe a method for the genetic modification of HESCs using electroporation, the preferred method for introduction of DNA into cells in which the desired outcome is gene targeting. This report provides methods for cell amplification, electroporation, colony selection and screening. The protocol we describe has been tested on four different HESC lines, and takes approximately 4 weeks from electroporation to PCR screening of G418-resistant clones.

PubMed Disclaimer

LinkOut - more resources