doi: 10.1038/nbt1301.
Epub 2007 Apr 22.
Quantitative proteomic assessment of very early cellular signaling events
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- PMID: 17450129
- DOI: 10.1038/nbt1301
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Quantitative proteomic assessment of very early cellular signaling events
Nat Biotechnol.
2007 May.
Free article
Abstract
Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.
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