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. 2007 May 21;96(10):1554-9.
doi: 10.1038/sj.bjc.6603765. Epub 2007 Apr 24.

Human papillomavirus in high- and low-risk areas of oesophageal squamous cell carcinoma in China

K Shuyama  1 , A Castillo, F Aguayo, Q Sun, N Khan, C Koriyama, S Akiba
Affiliations

Human papillomavirus in high- and low-risk areas of oesophageal squamous cell carcinoma in China

K Shuyama et al. Br J Cancer. .

Abstract

To examine the potential roles of human papillomavirus (HPV) in oesophageal squamous cell carcinoma (ESCC) development, we examined the presence of HPV DNA in paraffin-embedded ESCC tissues collected from two areas with different ESCC incidence rates in China, that is, Gansu (n=26) and Shandong (n=33), using PCR with SPF10 primers, or PCR with GP5+/GP6+ primers combined with Southern blot hybridisation. HPV genotype was determined by the INNO-LiPA HPV genotyping kit. HPV DNA was detected in 17 cases (65%) in Gansu, where ESCC incidence is much higher than in Shandong, where HPV was positive in two samples (6%). HPV genotypes 16 and 18 were detected in 79 and 16% of HPV-positive samples, respectively. Real-time PCR analysis suggested the presence of integrated form of HPV DNA in all the HPV-16-positive samples, but its viral load was estimated to be only <1-2 copies cell(-1). We could not detect HPV 16/18 E6 protein expression by immunostaining in any of the HPV-16-positive samples. Neither p16(INK4a) nor p53 expression was related to HPV presence in ESCCs. Further studies seem warranted to examine the possible aetiological roles of HPV in ESCC.

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Figures

Figure 1
Figure 1
Representative examples of INNO-LiPA HPV genotyping assay. Strip 1: positive control of HPV types 6 and 18; strip 2: negative control; strips 3–9 are ESCC samples; strip 3: HPV 16; strip 4: HPV 18; strip 5: HPV 16; strip 6: HPV 6 and 16; strip 7: HPV 16 and 18; strip 8: HPV 16 and strip 9: HPV 16 and 51.
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