doi: 10.1080/15257770701257277.
A novel universal linker for efficient synthesis of phosphorothioate oligonucleotides
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- PMID: 17454735
- DOI: 10.1080/15257770701257277
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A novel universal linker for efficient synthesis of phosphorothioate oligonucleotides
Nucleosides Nucleotides Nucleic Acids.
2007.
Abstract
A versatile and conformationally preorganized universal linker molecule is reported here for efficient synthesis of phosphorothioate oligonucleotides. With respect to nucleoside loaded support, comparable yield and quality based on ion-pair LC-MS are obtained for both deoxy and 2'-O-methoxyethyl modified phosphorothioate oligonucleotides. No 3'-phosphate or phosphorothioate monoester or any modification of universal molecule still attached to oligonucleotide was observed. [structure: see text]
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