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. 2007 Sep;34(9):681-5.
doi: 10.1097/01.olq.0000258307.18831.f0.

Performance of focus ELISA tests for HSV-1 and HSV-2 antibodies among university students with no history of genital herpes

Hayley D Mark  1 , Joy P Nanda, Jessica Roberts, Anne Rompalo, Johan H Melendez, Jonathan Zenilman
Affiliations

Performance of focus ELISA tests for HSV-1 and HSV-2 antibodies among university students with no history of genital herpes

Hayley D Mark et al. Sex Transm Dis. 2007 Sep.

Erratum in

  • Sex Transm Dis. 2007 Oct;34(10):835

Abstract

Objectives: To define the performance characteristics of the Focus ELISA HSV-1 and HSV-2 assay among 100 university students.

Study design: HSV-1 and HSV-2 Focus ELISA and Western Blot assays were performed on sera from university students who reported no history of genital herpes.

Results: HSV-2 and HSV-1 seroprevalence by Western Blot were 3.4% and 48%, respectively. In this population, the positive predictive value of the Focus HSV-2 ELISA was 37.5%, the sensitivity was 100%, and specificity was 94.1%. The PPV of the Focus HSV-1 ELISA was 96.7%, the sensitivity was 69.0%, and the specificity was 97.8%.

Conclusions: In this low-prevalence population, the positive predictive value of the Focus HSV-2 ELISA test was low. This finding, together with those reported elsewhere, indicates that caution is warranted when recommending HSV screening in low-prevalence or heterogeneous populations. Consideration should be given to raising the cutoff index value for defining a positive test result.

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References

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