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. 2007 Mar 28;13(12):1851-4; discussion 1854-6.
doi: 10.3748/wjg.v13.i12.1851.

Contribution of mononuclear bone marrow cells to carbon tetrachloride-induced liver fibrosis in rats

Bao-Qiang Cao  1 , Ji-Zong Lin, Yue-Si Zhong, Shao-Bin Huang, Nan Lin, Zhao-Feng Tang, Rui Chen, Peng Xiang, Rui-Yun Xu
Affiliations

Contribution of mononuclear bone marrow cells to carbon tetrachloride-induced liver fibrosis in rats

Bao-Qiang Cao et al. World J Gastroenterol. .

Abstract

Aim: To study the inhibitory effect of mononuclear bone marrow cell (BMC) transplantation on carbon tetrachloride (CCl(4)) -induced liver fibrosis in rats.

Methods: Rat liver fibrosis models were induced by CCl(4) and alcohol administration. After 8 wk, twenty rats were randomly allocated into treatment group (n = 10) and control group (n = 10). BMC were infused into the rats in treatment group via the portal vein, while heparinized saline was infused in control group. CCl(4) was hypodermically injected into the rats twice a week for 4 wk. At the end of wk 12, all rats were humanely sacrificed. Liver samples were taken and stained with HE or Masson trichrome. The general conditions, liver fibrosis (hydroxyproline and collagen fibre) and liver pathological grades in rats were evaluated.

Results: The general conditions of the rats in treatment group improved markedly, but not in control group. Hydroxyproline was 504.6 +/- 128.8 microg/g in treatment group, and 596.0 +/- 341.8 microg/g in control group. The percentage of collagen fibre was 3.75% +/- 0.98% in treatment group and 5.02% +/- 0.44% in control group. There was a significant difference between the two groups (P < 0.05). Liver pathological grade decreased from grade IV to grade III partially in treatment group (P < 0.05) with no obvious improvement in control group (P > 0.05). There was a significant difference between treatment group and control group (P < 0.05).

Conclusion: Transplantation of BMC can improve liver fibrosis due to chronic liver injury in rats.

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Figures

Figure 1
Figure 1
Fibrosis model protocol and BMC therapy for liver cirrhosis in rats.
Figure 2
Figure 2
Average weekly body mass of rats.
Figure 3
Figure 3
Histopathological changes of liver fibrosis 2 (A), 4 (B), 6 (C), 8 wk (D) after CCl4 injection, 4 wk after BMC transplantation in treatment group (E) and 4 wk after saline transplantation in control group (F) (HE, × 100).
See this image and copyright information in PMC

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