Transplantation of human embryonic stem cell-derived oligodendrocyte progenitors into rat spinal cord injuries does not cause harm
- PMID: 17465839
- DOI: 10.2217/17460751.1.4.469
Transplantation of human embryonic stem cell-derived oligodendrocyte progenitors into rat spinal cord injuries does not cause harm
Abstract
Demyelination contributes to loss of function following spinal cord injury. We have shown previously that transplantation of human embryonic stem cell-derived oligodendrocyte progenitors into adult rat 200 kD contusive spinal cord injury sites enhances remyelination and promotes recovery of motor function. Previous studies using oligodendrocyte lineage cells have noted a correlation between the presence of demyelinating pathology and the survival and migration rate of the transplanted cells. The present study compared the survival and migration of human embryonic stem cell-derived oligodendrocyte progenitors injected 7 days after a 200 or 50 kD contusive spinal cord injury, as well as the locomotor outcome of transplantation. Our findings indicate that a 200 kD spinal cord injury induces extensive demyelination, whereas a 50 kD spinal cord injury induces no detectable demyelination. Cells transplanted into the 200 kD injury group survived, migrated, and resulted in robust remyelination, replicating our previous studies. In contrast, cells transplanted into the 50 kD injury group survived, exhibited limited migration, and failed to induce remyelination as demyelination in this injury group was absent. Animals that received a 50 kD injury displayed only a transient decline in locomotor function as a result of the injury. Importantly, human embryonic stem cell-derived oligodendrocyte progenitor transplants into the 50 kD injury group did not cause a further decline in locomotion. Our studies highlight the importance of a demyelinating pathology as a prerequisite for the function of transplanted myelinogenic cells. In addition, our results indicate that transplantation of human embryonic stem cell-derived oligodendrocyte progenitor cells into the injured spinal cord is not associated with a decline in locomotor function.
Similar articles
-
Transplantation of oligodendrocyte precursor cells improves myelination and promotes functional recovery after spinal cord injury.Injury. 2012 Jun;43(6):794-801. doi: 10.1016/j.injury.2011.09.013. Epub 2011 Oct 20. Injury. 2012. PMID: 22018607
-
Human embryonic stem cell-derived oligodendrocyte progenitors for the treatment of spinal cord injury.Transpl Immunol. 2005 Dec;15(2):131-42. doi: 10.1016/j.trim.2005.09.007. Epub 2005 Nov 8. Transpl Immunol. 2005. PMID: 16412957 Review.
-
Human embryonic stem cell-derived oligodendrocyte progenitor cell transplants remyelinate and restore locomotion after spinal cord injury.J Neurosci. 2005 May 11;25(19):4694-705. doi: 10.1523/JNEUROSCI.0311-05.2005. J Neurosci. 2005. PMID: 15888645 Free PMC article.
-
Significance of remyelination by neural stem/progenitor cells transplanted into the injured spinal cord.Stem Cells. 2011 Dec;29(12):1983-94. doi: 10.1002/stem.767. Stem Cells. 2011. PMID: 22028197
-
Remyelination after spinal cord injury: is it a target for repair?Prog Neurobiol. 2014 Jun;117:54-72. doi: 10.1016/j.pneurobio.2014.02.006. Epub 2014 Feb 28. Prog Neurobiol. 2014. PMID: 24582777 Review.
Cited by
-
Cellular treatments for spinal cord injury: the time is right for clinical trials.Neurotherapeutics. 2011 Oct;8(4):704-20. doi: 10.1007/s13311-011-0076-7. Neurotherapeutics. 2011. PMID: 22002087 Free PMC article. Review.
-
Neglected No More: Emerging Cellular Therapies in Traumatic Injury.Stem Cell Rev Rep. 2021 Aug;17(4):1194-1214. doi: 10.1007/s12015-020-10086-7. Epub 2021 Jan 8. Stem Cell Rev Rep. 2021. PMID: 33420590 Free PMC article. Review.
-
Neural tissue engineering using embryonic and induced pluripotent stem cells.Stem Cell Res Ther. 2011 Apr 15;2(2):17. doi: 10.1186/scrt58. Stem Cell Res Ther. 2011. PMID: 21539726 Free PMC article. Review.
-
Achieving stable human stem cell engraftment and survival in the CNS: is the future of regenerative medicine immunodeficient?Regen Med. 2011 May;6(3):367-406. doi: 10.2217/rme.11.22. Regen Med. 2011. PMID: 21548741 Free PMC article.
-
Stem Cells Therapy for Spinal Cord Injury.Int J Mol Sci. 2018 Mar 30;19(4):1039. doi: 10.3390/ijms19041039. Int J Mol Sci. 2018. PMID: 29601528 Free PMC article. Review.
Publication types
MeSH terms
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical