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. 2007 Mar-Apr;43(2):193-8.

[Immobilization of Rhodococcus ruber strain gt1, possessing nitrile hydratase activity, on carbon sorbents]

[Article in Russian]
A Iu Maksimov, Iu G Maksimova, M V Kuznetsova, V F Olontsev, V A Demakov
  • PMID: 17476805

[Immobilization of Rhodococcus ruber strain gt1, possessing nitrile hydratase activity, on carbon sorbents]

[Article in Russian]
A Iu Maksimov et al. Prikl Biokhim Mikrobiol. 2007 Mar-Apr.

Abstract

Rhodococcus ruber strain gtl, possessing nitrile hydratase activity, was immobilized by adsorption on carbon supports differing in structure and porosity. The adsorption capacity of the supports towards cells, the substrate of the nitrile hydratase reaction (acrylonitrile), and the product (acrylamide) was studied. Also, the effect of immobilization and nitrile hydratase activity of bacteria was investigated, and the operational stability of the immobilized biocatalyst was determined. It was shown that crushed and granulated active coals were more appropriate for immobilization than fibrous carbon adsorbents.

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