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. 2007 Oct;9(5):369-72.
doi: 10.1016/j.jfms.2007.03.002. Epub 2007 May 2.

The detection of feline coronaviruses in blood samples from cats by mRNA RT-PCR

Kezban Can-Sahna  1 , Veysel Soydal Ataseven, Dilek Pinar, Tuba Ciğdem Oğuzoğlu
Affiliations

The detection of feline coronaviruses in blood samples from cats by mRNA RT-PCR

Kezban Can-Sahna et al. J Feline Med Surg. 2007 Oct.

Abstract

In this study, 26 blood samples were collected from 25 healthy cats and one cat with clinical signs suggestive of feline infectious peritonitis (FIP), namely, fever, weight loss, enlarged abdomen, and ascites. Blood samples were tested for feline coronavirus (FCoV) messenger RNA (mRNA) by an reverse transcriptase-polymerase chain reaction (RT-PCR) assay which has previously been described to have a high specificity in the diagnosis of clinical FIP [Simons AF, Vennema H, Rofina JE, Pol JM, Horzinek MC, Rottier PJM, Egberink HF (2005) A mRNA PCR for the diagnosis of feline infectious peritonitis. Journal of Virological Methods124, 111-116]. Overall we found 14 (54%) of the cats were positive for FCoV including the cat with clinical disease, but the high rate of positivity among healthy cats suggested a poor specificity for the clinical diagnosis of FIP among these cats. It was observed that the positivity rate was highest in cats aged between 6 months-1 year old. Our findings suggest that FCoVs may be present in the blood samples from healthy cats as well as cats with clinical FIP.

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Figures

Fig 1.
Fig 1.
M: 100 bp molecular weight marker (Fermentas), lanes 1 and 3: amplification mRNA RT-PCR product (295 bp) from blood samples, lanes 2, 4 and 6: GAPDH mRNA positive, lane 5: mRNA negative PCR control.
See this image and copyright information in PMC

References

    1. Addie D.D., Jarrett O. Feline coronavirus antibodies in cats, Veterinary Record 131, 1992, 202–203. - PubMed
    1. Addie D.D., Toth S., Herrewegh A.A.P.M., Jarrett O. Feline coronavirus in the intestinal content of cats with feline infectious peritonitis, Veterinary Record 139, 1996, 522–523. - PubMed
    1. Benetka V., Kübber-Heis A., Kolodziejek J., Nowotny N., Hofmann-Parisot M., Möstl K. Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis, Veterinary Microbiology 99, 2004, 31–42. - PMC - PubMed
    1. Campolo M., Desario C., Ricci D., Elia G., Decaro N., Martella V., Tempesta M., Di Trani L., Cavaliere N., Buonavoglia C. Identification of a feline coronavirus type I strain from a cat with feline infectious peritonitis by RT-PCR and phylogenetic analysis, New Microbiologica 28, 2005, 127–134. - PubMed
    1. Foley J.E., Poland A., Carlson J., Pedersen N.C. Risk factors for feline infectious peritonitis among cats in multiple-cat environments, Journal of American Veterinary Association 210, 1997, 1313–1318. - PubMed

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